COLD STORAGE OF IN VITRO RUBUS GERMPLASM

III Vitro Germplasm Conservation of Atropa baetica by Cold Storage

Gerlllplaslll 01" ;/1 v;tro derived plantlels of Atroptl baeticfl was lIlainlained by cold storage al 4°C in darkness for six 1Il0nths. Tite response of Ihe cold stored gcrlllplasm to growing culture co nditions was sludied on Murasltige & Skoog mediullI supplelllented wilh BAP (0.66 mg r ), NAA (0.24 mg r\ inosilol (100 mg r ), Ihiamine (1 mg 1'1) :Hul 3% sucrose durillg a six month periodo Tl...

Development of a multiplexed microsatellite set for fingerprinting red raspberry (Rubus idaeus) germplasm and its transferability to other Rubus species

This study aimed to develop a standardised and cost effective protocol for fingerprinting red raspberry germplasm using microsatellite (SSR) markers. Twenty SSRs well distributed through the raspberry genome were screened on 16 genetically diverse cultivars to assess polymorphism, allele size range, quality of amplification, ease of scoring, and discriminating power (DF). We selected published ...

Genetic variation of germination cold tolerance in Japanese rice germplasm

Low temperatures at the initial stages of rice development prevent fast germination and seedling establishment and may cause significant productivity losses. In order to develop rice cultivars exhibiting cold tolerance, it is necessary to investigate genetic resources, providing basic knowledge to allow the introduction of genes involved in low temperature germination ability from accessions in...

Effects of abscisic acid mannitol and temperature on invitro storage of potato germplasm

In vitro Plant Regeneration of Helianthus Annuus (Hyb. Azargol) From Alginate-Encapsulated Shoot Tips for Short Term Storage, Germplasm Exchange and Distribution

The present study demonstrates the potential of nutrient-alginate encapsulation of shoot tips of sunflower, Helianthus annuus (hyb. Azargol) for synthetic seed technology, which could be useful in germplasm distribution and exchange. Shoot tips from in vitro shoot cultures derived from mature seed explants were encapsulated in 3% sodium alginate and 100 mM CaCl2. 2H...